ASSESMENT OF SELENIUM STATUS OF HEALTHY ADULTS IN SOUTH WESTERN NIGERIA
Keywords:
Blood Selenium, Glutathion peroxidase, Selenium status, Biochemical markers
Abstract
Blood selenium (BSe) and plasma glutathione peroxidase (pIGSH-Px) activity were measured as biochemical markers of selenium status of 88 apparently healthy subjects (40 males; 48 females). The subjects were recruited from Abeokuta and Ibadan (South-Western Nigeria).
The mean age of the healthy subjects was 37.8 ± 8.6 (range 18 - 52) years. Their weight and height were measured and their body mass indices (BMI) computed. BSe was determined by atomic absorption spectrophotometry (AAS) while pIGSHPx activity was measured by spectrophotometric method.
The mean BSe concentration in healthy adults was found to be 0.188 + 0.026 mg/L while the mean pIGSH-Px activity was 0.127 ± 0.022 U/mL. These values were compared with some of those that were reported from other parts of the world.
It is evident from these results that there is no evidence of selenium deficiency in this environment since the range of BSe values found in healthy subjects is comparable to those found in similar populations in many areas of the world where selenium concentration in the soil and in human diet is considered to be adequate.
References
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McCoy, K.E. M., and Weswig, P. H.1969. Some selenium responses in rat not related to Vitamin E. J Nut. 98,383-390.
Moore, J.A., Noiva, R., and Wells, I.C. 1984. Selenium concentrations in plasma of patients with arteriographically defined coronary artherosclerosis. Clin. Chem., 30(7): 1171-1173.
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degeneration. J. Am. Chem. Soc., 79: 3292-3293.
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Taussky H.H 1956. A modified alkaline picrate method of determination of creatinine in serum.
Clin. Chim. Acta. 1: 200-202
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335-342.
Thomson C. D. Rea, H. M., Doesburg V. M. and Robinson M. F. 1977. Selenium concentration and glutathione peroxidase activities in whole blood of New Zealand residents Br. J. Nutr., 37 457-460
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Proc. Nutr. Soc. 41: 20-23.
Wastermarck, T., Raunu, P., Kirjarinta, M., and Lappalainen, L. 1. 1977. Selenium content of whole blood and serum in adults and children of different ages from different parts of Finland, Acta.
Pharmucol, Toxicol. 40, 465-475.
Yang, G., Wang, Z., Zhou, R. and Sun, S.I. 1983. Endemic selenium intoxication of human in China
Am. J.Clin. Nutr. 37, 872-876.
Zagrodzki A. D., Szmigiel, H., Ratajczak, R.. Szybinski, Z. and Zachwiega, Z. .2000. The role of selenium in iodine metabolism in children with
goiter. Environmental Health Perspectives 108, ( I ), 67-71.
Allaway, W. H., Kubota, J., Losee, F., and Roth, M. 1968. Selenium, Molybdenum, and Vanadium in human blood. Arch. Environ. Health, 16: 342-348.
Arthur, J. R. and Beckett, G. J. 1994. Newer aspect of micronutrients in at risk Groups: New metabolic role for selenium. Proc. Nut. Soc. 53: 615 - 624.
Burk R.F., Hill K.E, Read R and Bellow T 1991. Response of rat selenoprotein -P to selenium administration and fate of its selenium. Am. J. Physiol. 261: 26 - 30
Calvin, H. L, Grosshans K., Musicant - Shikora, S. R. and Turner, S. 1.1987. A developmental study of ratsperm and testis selenoproteins. J. Reprod Fert. 81, 1-7.
Coulombe J. S, and Favreau L.A 1963. A simple colorimetric method for the determination of urea in the presence of diacetyl monoxime and thiosemicarbazide. Clin. Chem. 9 : 102 -104.
Dickson, R.C. and Tomlinson, R.H. 1967. Selenium in blood and human tissues. Clin.Chim. Acta, 16: 311-321.
Henry R. J., Sobel S and Kim J. 1957. A phosphotungistic acid method for the determination of uric acid in biological fluids. Amer. J. Clin. Path. 28: 152 - 154
Hill K. E and Burk R. F 1994. Selenoprotein P- an extra cellular protein containing multiple selenocysteins. In selenium in Biology And Human Health (R.F Burk, Editor). New York : Springer Verlag pp 117-131
Howe, M. 1974. Selenium in the blood of South Dakotans. Arch. Environ. Health 34:444 - 448.
lloyd, B. Lloyd, R. S., and Clayton, B. E. , 1983. Effect of Smokings alcohol and other factors on the selenium status of a healthy population, J. Epidenniol, Commun. Health 57, 213.
Kumpulainen J., Vuori E. and Siimes M. A., 1985. Effect of maternal dietary selenium intake on selenium level in breast milk. Int. J. Vitamin Nun•. Res. 54 , 251-253.
Lawrence, R.A. and Burk, R.F. 1978. Species, tissue, and subcellular distribution of non Se-dependent glutathione peroxidase activity. J. Nutr., 108:21 1-215.
Lawrence, R. A. and Burk, R. F.1976. Glutathione. Peroxidase activity in selenium deficiency rat liver, Biochem. Biophys. Res. Commun. 71, 952-558.
Lockitch G. 1989. Selenium, clinical significance and analytical concepts Cri. Rev.Clin. Lab. Sc. 27, 483-542.
McCoy, K.E. M., and Weswig, P. H.1969. Some selenium responses in rat not related to Vitamin E. J Nut. 98,383-390.
Moore, J.A., Noiva, R., and Wells, I.C. 1984. Selenium concentrations in plasma of patients with arteriographically defined coronary artherosclerosis. Clin. Chem., 30(7): 1171-1173.
Oster, O. Prellwitz, W.; Kasper, W and Meinertz, T.1983. Congestive cardiomyopathy and selenium content of serum. Clin. Chim. Acta. 128, 125-132.
Paglia D. and Valentine W'.N. 1967. Study on the quantitative and qualitative characterization of erythrocyte glutathione peroxides J. Lab. Clin Med. 70 154 -169
Pleban, P.A., Munyani, A., and Beachum, J. 1982. Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes, 28:311-3 16. Clin. Chem
Rea, H.M., Thomson, C.D., Campbell, D.R., and Robinson, M.F. 1979. Relation between blood selenium concentrations and glutathione peroxidase (EC 1.11. 1.9) activities of New Zealand residents
and visitors to New Zealand. Br. J. Nutr., 42: 201¬208.
Robinson, and Thompson, 1981. Selenium levels in humans vs environmental sources. Selenium in Biology and Medicine, Westport, Conneticut, The AVI Publishing Co. Inc. pp 283-302.
Schultz, T.D. and Leklem, J. E. 1983. Selenium status of vegetarians, nonvegetarians, and hormone¬dependent cancer subjects. Am. J. Clin. Nuir., 37:
114-118.
Schwarz, K. and Foltz, C. M. 1957. Selenium as an integral part of Factor 3 against dietary necrotic liver
degeneration. J. Am. Chem. Soc., 79: 3292-3293.
Spangnolo, A., Morisi, G., Marano, G., Righetti, G., Masetta, A. Menotti, A. 1991. Bloodselenium and precursor of cardiovascular risk factor in adolescents
Eur J. Epidemiol 7: 654 - 657.
Taussky H.H 1956. A modified alkaline picrate method of determination of creatinine in serum.
Clin. Chim. Acta. 1: 200-202
Thompson, J. N. and Scott, M. L. 1969. Role of selenium in the nutrition of the chick. J. Nutr., 97:
335-342.
Thomson C. D. Rea, H. M., Doesburg V. M. and Robinson M. F. 1977. Selenium concentration and glutathione peroxidase activities in whole blood of New Zealand residents Br. J. Nutr., 37 457-460
Ward, K, Anderson. J. Arthur, J. R., Aggett, P. J. 1982. Selenium status in healthy and disease states.
Proc. Nutr. Soc. 41: 20-23.
Wastermarck, T., Raunu, P., Kirjarinta, M., and Lappalainen, L. 1. 1977. Selenium content of whole blood and serum in adults and children of different ages from different parts of Finland, Acta.
Pharmucol, Toxicol. 40, 465-475.
Yang, G., Wang, Z., Zhou, R. and Sun, S.I. 1983. Endemic selenium intoxication of human in China
Am. J.Clin. Nutr. 37, 872-876.
Zagrodzki A. D., Szmigiel, H., Ratajczak, R.. Szybinski, Z. and Zachwiega, Z. .2000. The role of selenium in iodine metabolism in children with
goiter. Environmental Health Perspectives 108, ( I ), 67-71.
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